Regulatory
Part:BBa_K1484214:Design
Designed by: Salil Bhate Group: iGEM14_Cambridge-JIC (2014-10-08)
MpEF1a (Marchantia poylmorpha Elongation Factor 1, PlantSyntax Pro+5U)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 146
Illegal BglII site found at 234
Illegal XhoI site found at 1479 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 858
Illegal NgoMIV site found at 922
Illegal NgoMIV site found at 1064 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1
Illegal BsaI.rc site found at 1751
Illegal SapI.rc site found at 1350
Design Notes
Transcription start site unknown. This contains the 5'UTR of the EF1a gene.
Restriction sites added to enable PlantSyntax compatible assembly
Source
This promoter was discovered by sequence comparison with EF1 type promoters in P. patens and A. thaliana. This version was isolated by PCR from genomic DNA, and modified by to remove illegal restriction sites.